@article{TCR1342,
author = {Rajiv Kumar and Houari Korideck and Wilfred Ngwa and Ross I. Berbeco and G. Mike Makrigiorgos and Srinivas Sridhar},
title = {Third generation gold nanoplatform optimized for radiation therapy},
journal = {Translational Cancer Research},
volume = {2},
number = {4},
year = {2013},
keywords = {},
abstract = {We report the design and fabrication of third generation ultrasmall PEGylated gold nanoparticles based platform (AuRadTM) optimized for applications in radiation therapy. The AuRadTM nanoplatform has the following key features: (I) surface coating of hetero-bifunctional-PEG with amine, carboxyl, methoxy functional groups, which make this a versatile nanoplatform to conjugate various moieties like fluorophores, peptides, drugs, radiolabels; (II) size that is optimized for longer circulation, higher tumor uptake and modulated clearance; (III) high radiation enhancement. We have synthesized ultrasmall 2-3 nm gold nanoparticles, followed by attachment of hetero-bifunctional PEG and further conjugation of fluorophore AlexaFlour 647 for optical imaging, with a stability of more than 6 months. Confocal bioimaging with HeLa cells showed robust uptake of biocompatible nanoparticles in cells. Irradiation experiments X-rays showed greater than 2.8-fold cell kill enhancement as demonstrated by clonogenic survival assays. The results indicate that AuRad nanoplatform can act as potential theranostic agent in radiation therapy.},
issn = {2219-6803}, url = {https://tcr.amegroups.org/article/view/1342}
}