@article{TCR15156,
author = {Can Cui and Jianhui Wu and Xiaojing Cheng and Liang Yan and Xiaoya Guan and Bin Dong and Min Zhao and Xiuyun Tian and Chunyi Hao},
title = {LAPTM4B-35 expression was correlated with favorable postoperation survival in pancreatic cancer patients},
journal = {Translational Cancer Research},
volume = {6},
number = {4},
year = {2017},
keywords = {},
abstract = {Background: Pancreatic cancer (PC) is malignant and lethal with its invasiveness and metastasis. Till now, it’s still very difficult to diagnose PC at its early stage due to lack of obvious symptoms. Therefore, there is an urgent need to find an efficient biomarker to help diagnose PC as early as possible. Lysosome-associated protein transmembrane 4 beta-35 (LAPTM4B-35) is a kind of transmembrane oncoprotein which has been proved to be overexpressed in a variety of solid tumors. Few studies have focused on the expression of LAPTM4B-35 and its function in PC patients. This study aimed to assess LAPTM4B-35 expression in PC and to investigate the relationship between LAPTM4B-35 expression with clinicopathological features and post-operation survival of PC patients.
Methods: We collected tumor tissues and paired non-cancerous tissues from 93 PC patients and evaluated LAPTM4B-35 expression through immunohistochemistry assay. And we also explored LAPTM4B-35 expression of mRNA and protein by means of reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR and Western blotting in PC cell lines and frozen tumor tissues. We used chi-squared test and Fisher’s exact test to evaluate the relationship between LAPTM4B-35 expression and clinicopathological factors. Kaplan-Meier survival analysis and Cox regression were used for survival analysis. A two-sided P value less than 0.05 was considered to be statistically significant.
Results: LAPTM4B-35 was found to be expressed in 60 of 93 (64.5%) tumor tissues and 7 of 93 (7.5%) paired non-cancerous tissues (P},
issn = {2219-6803}, url = {https://tcr.amegroups.org/article/view/15156}
}