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siRNA-mediated silencing of FOXQ1 expression inhibits cell proliferation and induces apoptosis in human osteosarcoma cells

   
@article{TCR16913,
	author = {Hao Wu and Liming Xia},
	title = {siRNA-mediated silencing of FOXQ1 expression inhibits cell proliferation and induces apoptosis in human osteosarcoma cells},
	journal = {Translational Cancer Research},
	volume = {6},
	number = {6},
	year = {2017},
	keywords = {},
	abstract = {Background: This study aimed to explore the expression of forkhead box Q1 (FOXQ1) and its effects on proliferation and apoptosis in human osteosarcoma cell line U2OS. 
Methods: The expression of FOXQ1 was detected by Western blotting in osteosarcoma cell line U2OS. U2OS cells were divided into three groups: experimental group (FOXQ1-siRNA group), negative control group (NC-siRNA group) and blank group (untransfection group). FOXQ1-targeted siRNA and control siRNA were transfected into U2OS cells in FOXQ1-siRNA group and NC-siRNA group, respectively. The mRNA and protein levels of FOXQ1 were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. U2OS cell proliferation was detected by MTT assay and apoptosis was measured by flow cytometry. 
Results: FOXQ1-targeted siRNA transfection significantly inhibited FOXQ1 gene expression at both mRNA and protein levels (P},
	issn = {2219-6803},	url = {https://tcr.amegroups.org/article/view/16913}
}