@article{TCR7251,
author = {Peng Yuan and Xiaojing Cheng and Xiaojiang Wu and Lin Li and Lianhai Zhang and Ziyu Li and Xiaofang Xing and Hong Du and Xiaohong Wang and Ying Hu and Qingyun Zhang and Guoren Deng and Xianzi Wen and Jiafu Ji},
title = {OSMR and SEPT9: promising biomarkers for detection of colorectal cancer based on blood-based tests},
journal = {Translational Cancer Research},
volume = {5},
number = {2},
year = {2016},
keywords = {},
abstract = {Background: Early screening and monitoring of the stage of colorectal cancer (CRC) is crucial for successful treatment and patient survival. Our purpose was to identify that evaluating methylated oncostatin M receptor (mOSMR) and Septin 9 (mSEPT9) in plasma DNA could be a feasible method for CRC diagnosis.
Methods: Plasma samples (40 samples have matched cancer and adjacent non-cancer tissues) were collected from 187 patients with CRC, 25 patients with polyps and 109 healthy controls. qRT-PCR assay was used to analyze SEPT9 and OSMR mRNA levels. DNA bisulfite treatment, the levels of mSEPT9 and mOSMR in CRC tissues and plasma samples were evaluated by using fluorescence-based real-time PCR assay (MethyLight). mSEPT9 and mOSMR levels in plasma were compared before and after curative resection. Serum CEA, CA19-9, CA72-4 and CA242 were also measured and analyzed in parallel.
Results: SEPT9 and OSMR mRNA levels were significantly lower in CRC tissues compared with those in their adjacent noncancerous tissues (P=0.006 and 0.002, respectively). mSEPT9 and mOSMR were significantly higher in CRC tissues compared with those in their adjacent non-cancerous tissues (P},
issn = {2219-6803}, url = {https://tcr.amegroups.org/article/view/7251}
}