@article{TCR7261,
author = {Liu Yang and Haitao Zhang and Qi Yao and Yaohua Yan and Jianguo Chen and Ronghua Wu and Mei Liu},
title = {miR-17 enhances proliferation and migration and inhibits apoptosis in glioma cells by regulating SASH1 expression},
journal = {Translational Cancer Research},
volume = {5},
number = {2},
year = {2016},
keywords = {},
abstract = {Background: To investigate the mechanisms underlying the regulation of human glioma cell growth, proliferation, and apoptosis by microRNA-17 (miR-17) to provide targets for novel human glioma therapies.
Methods: The expression of miR-17 in human glioma tissues and cell lines was detected using real-time quantitative PCR (qRT-PCR). A miR-17 inhibitor was transfected into the U251 human glioma cell line using liposomes, and changes in miR-17 expression were detected using qRT-PCR. The effects of miR-17 on numerous biological characteristics of U251 cells, including viability, proliferation, apoptosis, and migration, were analyzed using MTT, flow cytometry, and Transwell migration chamber assays. A luciferase reporter gene system was used to validate SAM- and SH3-domain containing 1 (SASH1) as a true target of miR-17. The effects of miR-17 on SASH1 protein expression were determined using western blotting.
Results: qRT-PCR results showed that compared with adjacent tissues, miR-17 expression was significantly increased in human glioma tissues and cells lines (P},
issn = {2219-6803}, url = {https://tcr.amegroups.org/article/view/7261}
}