TY  - JOUR
AU  - Cheng, Zhuzhong
AU  - Jiang, Xiao
AU  - Yang, Li
AU  - Shen, Taipeng
AU  - Wang, Xiaoxiong
AU  - Lu, Hao
AU  - Wu, Jun
AU  - Xia, Chunchao
AU  - Song, Bin
AU  - Ai, Hua
PY  - 2016
TI  - Micro PET imaging of 18F-Fluoromisonidazole in an MDA-MB-231 triple negative human breast cancer xenograft model
JF  - Translational Cancer Research; Vol 5, No 3 (June 29, 2016): Translational Cancer Research
Y2  - 2016
KW  - 
N2  - Background: While 18F-Fluoromisonidazole (18F-FMISO) is the most frequently used positron emission tomography (PET) tracer for detecting hypoxia, it has not been studied in a metastatic triple-negative [lack expression of estrogen receptor (ER), progesterone receptor (PR) and human EGF receptor 2 (HER2)] human breast cancer cell xenografts model to our best knowledge. This study focuses on whether 18F-FMISO can detect the hypoxic status of triple-negative human breast cancer (TNBC).  Methods: The TNBC cells MDA-MB-231 were successfully inoculated in right forelimb of nude mice. Nude mice models with TNBC xenografts were assessed by micro-PET imaging with 18F-FMISO, and hypoxic status of the TNBC xenografts was determined by immunohistochemistry. We also compared 18F-FDG uptake, the most commonly used PET tracer in clinical practice, with 18F-FMISO uptake to find out its correlation in MDA-MB-231 xenografts.   Results: For 18F-FMISO, intestines and liver as well as bladder could be seen in micro-PET images. 18F-FDG showed physiologically high uptake in brain, heart, bladder and intestinal tracts. The quantitative radioactivity of 18F-FMISO and 18F-FDG in tumor were 2.18±0.15 and 3.84±0.54 %ID/g, respectively. The quantitative radioactivity of 18F-FMISO and 18F-FDG in muscle were 1.23±0.08 and 0.59±0.09 %ID/g, respectively. The tumor-to-muscle ratios were 1.79±0.015 and 7.11±2.84 for 18F-FMISO and 18F-FDG, respectively. Immunofluorescent images from MDA-MB-231 cryosections showed significant hypoxia.  Conclusions: 18F-FMISO PET may be used for detection of hypoxia in tumor microenvironment of triple negative human breast cancer.
UR  - https://tcr.amegroups.org/article/view/8161