Original Article

Downregulation of ZFX is associated with inhibition of prostate cancer progression by baicalein

Wenhui Zhu, Jimeng Hu, Mengbo Hu, Haowen Jiang


Background: Baicalein is a bioactive flavonoid that is widely used in China. However, its effects on prostate cancer (PCa) and relative mechanisms have not been extensively studied. We intended to study the potential anti-tumor effects of baicalein and the related mechanisms on PCa.
Methods: We analyzed the inhibitory effects of baicalein on PCa by utilizing a cell viability assay and an in vivo tumor growth assay. Wound healing assay and transwell assay were performed to investigate cancer cell migration and invasion abilities. Then the expressions of zinc finger protein X-linked (ZFX) in PCa cells and xenograft tumor were respectively detected by western blot and immunohistochemistry assay.
Results: Baicalein significantly inhibited cell proliferation in vitro and tumor growth of PCa in vivo. Consistently, baicalein induced significantly inhibition on the migration and invasion abilities of PCa cells. In addition, ZFX expressions were downregulated by baicalein in PCa both in vitro and in vivo.
Conclusions: Our study provides evidence that baicalein plays important roles in PCa therapy probably by downregulating ZFX expression, which was demonstrated to be involved in PCa progression, and thus may be developed as a novel and efficient candidate agent for PCa therapy.

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